A Canine Leptospirosis Clinical Case Due to Leptospira interrogans (Serogroup Icterohaemorrhagiae) in a Dog Kennel in Castelvetrano (Western Sicily, South Italy)

Leptospirosis is a worldwide widespread zoonosis caused by Leptospira genus. We report an acute leptospirosis case in puppy housed at municipal kennel and the subsequent diagnostic investigations carried out on all dogs kennel. Laboratory investigation included mainly microagglutination test, real-time PCR, multi-locus sequence typing (MLST) for Other agents of infection were excluded. The resulted positive interrogans Icterohaemorrhagiae both with serological molecular assays. All other 66 underwent clinical laboratory twice, 15 days apart. No dog showed signs. At first sampling, eight (12%) antibodies against serogroup Icterohaemorragiae serovar Copenhageni. Real-time PCR urine samples seropositive detected spp. DNA one sample, then identified as MLST. Fifteen after, four previous still collected from negative PCR. study allowed early confirmation identification least asymptomatic carrier pathogenic prompt activation appropriate management measures limiting extinguishing infection.